Exploring the Effect of Saliva Contamination on Shear Bond Strength of Dental Adhesives

Authors

  • Khalida Ehsan The Superior University and Shaikh Zayed Hospital ,Lahore, Pakistan.
  • Fahmoona Irfan University Medical and Dental College, Faisalabad ,Pakistan.
  • Ali Khan Memon Muhammad Dental College, Mirpurkhas, Sindh
  • Madiha Khalid Memon Mohammed Dental College,Ibn- e- Sina University ,Mirpurkhas, Pakistan.
  • Kaleem Memon Muhammad Dental College, Mirpurkhas, Sindh Pakistan.
  • Muhammad Arsalan Shah University of Florence ,Italy. https://orcid.org/0009-0009-1773-2623

DOI:

https://doi.org/10.36283/ziun-pjmd14-4/017

Keywords:

Dental Bonding, Dental Materials, Adhesives, Saliva, Shear Strength

Abstract

Background: Contamination of saliva during restorative procedures may be one of the most common clinical concerns in adhesive dentistry. It may interfere with adhesive systems-dentin interaction, and eventually lead to shear bond strength (SBS) loss and influence the restoration longevity. Systems that are generally employed are the etch-and-rinse and self-etch adhesives but sensitivity to salivary contamination is a major issue. The objective of the present study was to determine how saliva contamination influences the SBS of these adhesive systems.

Methods: This comparative in-vitro study was conducted on 120 human premolars that had been extracted. The teeth were randomly subdivided into four sets (n = 30 each): Group A (etch-and-rinse without contamination), Group B (etch-and-rinse with saliva contamination), Group C (self-etch without contamination), and Group D (self-etch with contamination). Preparation of composite build-ups was done then SBS testing done using universal testing machine. Failure modes were evaluated under the stereomicroscopy. Statistical analyses were ANOVA, independent t-tests, chi-square tests. The significant p-value was assumed to be less than 0.05.

Results: The maximum SBS was noted in etch-and-rinse that was not contaminated with saliva (Group A; 22.4 ± 2.8 MPa) and significantly lower in saliva-contaminated etch-and-rinse (14.1 ± 3.2 MPa, p = 0.001). The bond strength of self-etch adhesives was relatively lower in general (Group C: 18.3 ± 2.9 MPa), although contamination resulted in a weaker drop (Group D: 13.9 ± 2.7 MPa, p = 0.01). A failure mode analysis revealed that there were higher adhesive failures in contaminated groups (p = 0.002).

Conclusion: Saliva contamination greatly compromises the SBS of both adhesive systems with the etch-and-rinse more susceptible. Proper moisture management and selection of adhesives are very important to long-term restorative success.

Author Biographies

  • Khalida Ehsan, The Superior University and Shaikh Zayed Hospital ,Lahore, Pakistan.

    Department of Pathology,


  • Fahmoona Irfan, University Medical and Dental College, Faisalabad ,Pakistan.

    Department of Orthodontics,


  • Ali Khan Memon, Muhammad Dental College, Mirpurkhas, Sindh

    Department of Oral Pathology


  • Madiha Khalid Memon, Mohammed Dental College,Ibn- e- Sina University ,Mirpurkhas, Pakistan.

    Department of Oral Biology,


  • Kaleem Memon, Muhammad Dental College, Mirpurkhas, Sindh Pakistan.

    Department of Oral Pathology,


  • Muhammad Arsalan Shah, University of Florence ,Italy.

    Department of Biomedical Sciences,

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Published

2025-09-29

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How to Cite

1.
Ehsan K, Irfan F, Memon AK, Memon MK, Memon K, Shah MA. Exploring the Effect of Saliva Contamination on Shear Bond Strength of Dental Adhesives. PJMD [Internet]. 2025 Sep. 29 [cited 2026 Jun. 4];14(4). Available from: https://ojs.zu.edu.pk/pjmd/article/view/3685

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