Early CBC Screening for Detecting Subclinical Anemia in Adolescent Females

Authors

  • Saadia Saad United Medical and Dental College, Karachi, Pakistan.
  • Raheela Pyar Ali Husaini Institute of Hematology, Karachi, Pakistan.
  • Uzma Najam United Medical and Dental College, Karachi, Pakistan.
  • Uzma Shahid United Medical and Dental College, Karachi, Pakistan.
  • Asaad Javaid Mirza Baqai Medical University, Karachi, Pakistan.

DOI:

https://doi.org/10.36283/ziun-pjmd14-1/022

Keywords:

Anemia, Thalassemia, Iron Deficiency

Abstract

This cross-sectional study sought to assess the prevalence of subclinical anemia in adolescent females. It recruited a total of 118 adolescent females from 3rd-5th April 2024 via convenient sampling. The females who appeared healthy and had no known prior medical condition were screened for anemia. Demographics, medical history, and vital signs were recorded and CBC and peripheral blood smear tests were performed at Husaini Diagnostic Laboratory.  Using SPSS v.25, frequency and percentages for prevalence and Chi-square for association were employed with p<0.05 as significant. A substantial part of girls, 35 out of 118 (29.7%) were anemic. Underweight BMI was associated with a higher risk of anemia. Anemia severity was generally mild, with only 8 (6.8%) exhibiting moderate anemia. Microcytic hypochromic RBCs were observed in 23 out of 35 (65.7%) anemic cases, suggesting iron deficiency as the primary cause. However, serum ferritin and hemoglobin electrophoresis were recommended to confirm iron deficiency or thalassemia respectively.

Author Biographies

  • Saadia Saad, United Medical and Dental College, Karachi, Pakistan.

    Assistant Professor, Department of  Physiology 

  • Raheela Pyar Ali, Husaini Institute of Hematology, Karachi, Pakistan.

    Clinical Pathologist 

  • Uzma Najam, United Medical and Dental College, Karachi, Pakistan.

    Associate Professor, Department of Physiology, 

  • Uzma Shahid, United Medical and Dental College, Karachi, Pakistan.

    Professor, Department of Physiology, 

  • Asaad Javaid Mirza, Baqai Medical University, Karachi, Pakistan.

    Dean

References

Stahl-Gugger A, de Godoi Rezende Costa Molino C, Wieczorek M, Chocano-Bedoya PO, Abderhalden LA, Schaer DJ, Spahn DR, Orav EJ, Vellas B, Da Silva JA, Kressig RW. Prevalence and incidence of iron deficiency in European community-dwelling older adults: an observational analysis of the DO-HEALTH trial. Aging clinical and experimental research. 2022 Sep;34(9):2205-15.10.1007/s40520-022-02093-0.

Le CH. The prevalence of anemia and moderate-severe anemia in the US population (NHANES 2003-2012). PloS one. 2016 Nov 15;11(11):e0166635. doi: 10.1371/journal.pone.0166635.

Kumari R, Bharti RK, Singh K, Sinha A, Kumar S, Saran A, Kumar U. Prevalence of iron deficiency and iron deficiency anaemia in adolescent girls in a tertiary care hospital. Journal of clinical and diagnostic research: JCDR. 2017 Aug;11(8):BC04. doi:10.7860/JCDR/2017/26163.10325.

Kumar A, Sharma E, Marley A, Samaan MA, Brookes MJ. Iron deficiency anaemia: pathophysiology, assessment, practical management. BMJ open gastroenterology. 2022 Jan 1;9(1):e000759.https://doi.org/10.1136/bmjgast-2021-000759.

Das Mahapatra PP, Roy C, Agarwal K, Sharma SD, Chowdhury SR, Sharma S, Rajagopal H, Meher D. Non-invasive hemoglobin screening device: a promising digital method for reducing anemia prevalence through routine screening and timely intervention. Hematology. 2024 Dec31;29(1):2365078.https://doi.org/10.1080/16078454.2024.2365078

Gardner W, Kassebaum N. Global, regional, and national prevalence of anemia and its causes in 204 countries and territories, 1990–2019. Current Developments in Nutrition. 2020 Jun 1;4:nzaa053_035.https://doi.org/10.1093/cdn/nzaa053_035.

World Health Organization. Guideline on haemoglobin cutoffs to define anaemia in individuals and populations. World Health Organization; 2024 Mar 5. Available from: https://www.who.int/publications/i/item/9789240088542.

Guralnik J, Ershler W, Artz A, Lazo‐Langner A, Walston J, Pahor M, Ferrucci L, Evans WJ. Unexplained anemia of aging: Etiology, health consequences, and diagnostic criteria. Journal of the American Geriatrics Society. 2022 Mar;70(3):891https://doi.org/10.1111/jgs.17565.

Sari P, Judistiani RT, Herawati DM, Dhamayanti M, Hilmanto D. Iron Deficiency Anemia and Associated Factors Among Adolescent Girls and Women in a Rural Area of Jatinangor, Indonesia. International Journal of Women's Health. 2022 Dec 31:1137-47.https://doi.org/10.2147/IJWH.S376023

Clénin GE. The treatment of iron deficiency without anaemia (in otherwise healthy persons). Swiss medical weekly. 2017 Jun 14;147(2324):w14434-doi:10.4414/smw.2017.14434

Taha Z, Elhag H, Hassan AA, Adam I. Association Between Hemoglobin Level, Anemia, and Hypertension Among Adults in Northern Sudan: A Community-Based Cross-Sectional Study. Vascular Health and Risk Management. 2024 Dec 31:323-31.https://doi.org/10.2147/VHRM.S472480.

Low MS, Speedy J, Styles CE, De‐Regil LM, Pasricha SR. Daily iron supplementation for improving anemia, iron status and health in menstruating women. Cochrane Database of Systematic Reviews. 2016 April.https://doi.org/10.1002/14651858.CD009747.pub2.

Kamruzzaman M. Is BMI associated with anemia and hemoglobin level of women and children in Bangladesh: A study with multiple statistical approaches. PloS one. 2021 Oct 28;16(10):e0259116.https://doi.org/10.1371/journal.pone.0259116.

Eckhardt CL, Torheim LE, Monterrubio E, Barquera S, Ruel MT. The overlap of overweight and anaemia among women in three countries undergoing the nutrition transition. European journal of clinical nutrition. 2008 Feb;62(2):238-46.

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Published

2025-01-10

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How to Cite

1.
Saad S, Ali RP, Najam U, Shahid U, Mirza AJ. Early CBC Screening for Detecting Subclinical Anemia in Adolescent Females. PJMD [Internet]. 2025 Jan. 10 [cited 2026 Jun. 11];14(1):152-6. Available from: https://ojs.zu.edu.pk/pjmd/article/view/3310

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