Evaluating Neurotherapeutic Potential of Myricetin by In-Vivo research models
DOI:
https://doi.org/10.36283/ziun-pjmd14-4/034Keywords:
Nerve Growth Factor, Flavonoids, Rats, Wistar, Neurodegenerative DiseasesAbstract
Background: Oxidative stress, neuroinflammation, and poor neurotrophic signaling are associated with neurological diseases. Myricetin is a bioflavonoid present in berries, vegetables, and medicinal herbs, with strong anti-inflammatory, antioxidant, and neurotrophic properties in animal studies. The objective of the present in-vivo research was to assess the neuroprotective potential of myricetin on nerve growth factor (NGF) expression and neuroinflammation in chemically induced neural injury. Materials and Methods: Thirty healthy male adult Wistar rats (180-220g, 8 weeks old) were randomly divided into five groups (n=6 per group). Group I was used as a control, with no intervention. Groups II to V were exposed to a standard propionic acid (PPA) to induce neuroinflammation. Groups III-V were induced and subsequently administered with oral myricetin of 50 mg/kg, 100 mg/kg, and 200 mg/kg for 28 days. Serum levels of NGF were measured with Enzyme-linked Immunosorbent assay (ELISA). One-way ANOVA was performed for statistical analysis using SPSS. Results: PPA decreased NGF to 4.3 ± 0.5 pg/ml (p< 0.001). Myricetin restored NGF to 9.3, 7.6, and 9.8 pg/mL via 50, 100, and 200 mg/kg doses, respectively. At 200 mg/kg, C-reactive protein (CRP), tumor necrosis factor (TNF), and malondialdehyde (MDA) were decreased to 1.7 ± 0.2 mg/L, 26 ± 3 pg/mL, and 2.9 ± 0.3 nmol/mg, compared to 4.9 ± 0.4, 58 ± 4, and 6.7 ± 0.4. (p < 0.001). Conclusion: Myricetin exhibits promising neurotherapeutic potential, evidenced by its ability to upregulate NGF and mitigate neuroinflammatory damage, making it a potential therapeutic option for the treatment of neurological disorders.
References
1. Li J, Xiang H, Huang C, Lu J. Pharmacological Actions of Myricetin in the Nervous System: A Comprehensive Review of Preclinical Studies in Animals and Cell Models. Front Pharmacol. 2021 Dec 16;12:797298. doi: 10.3389/fphar.2021.797298.
2. Rushendran R, Begum RF, Singh S A, Narayanan PL, Vellapandian C, Prajapati BG, et al. Navigating neurological disorders: harnessing the power of natural compounds for innovative therapeutic breakthroughs. EXCLI J. 2024 Apr 23;23:534-569. doi: 10.17179/excli2024-7051.
3. Bellavite P. Neuroprotective Potentials of Flavonoids: Experimental Studies and Mechanisms of Action. Antioxidants (Basel). 2023 Jan 27;12(2):280. doi: 10.3390/antiox12020280.
4. Chen T, Hu Y, Lu L, Zhao Q, Tao X, Ding B, et al. Myricetin attenuates hypoxic-ischemic brain damage in neonatal rats via NRF2 signaling pathway. Front Pharmacol. 2023 Mar 8;14:1134464. doi: 10.3389/fphar.2023.1134464.
5. Boriero D, Carcereri de Prati A, Antonini L, Ragno R, Sohji K, Mariotto S, et al. The anti-STAT1 polyphenol myricetin inhibits M1 microglia activation and counteracts neuronal death. FEBS J. 2021 Apr;288(7):2347-2359. doi: 10.1111/febs.15577.
6. Shimada Y, Sato Y, Kumazoe M, Kitamura R, Fujimura Y, Tachibana H. Myricetin improves cognitive function in SAMP8 mice and upregulates brain-derived neurotrophic factor and nerve growth factor. Biochem Biophys Res Commun. 2022 Aug 6;616:33-40. doi: 10.1016/j.bbrc.2022.05.039.
7. Ma J, Liu J, Chen Y, Yu H, Xiang L. Myricetin Improves Impaired Nerve Functions in Experimental Diabetic Rats. Front Endocrinol (Lausanne). 2022 Jul 19;13:915603. doi: 10.3389/fendo.2022.915603.
8. Wang C, Ouyang S, Zhu X, Jiang Y, Lu Z, Gong P. Myricetin suppresses traumatic brain injury-induced inflammatory response via EGFR/AKT/STAT pathway. Sci Rep. 2023 Dec 20;13(1):22764. doi: 10.1038/s41598-023-50144-x.
9. Guo W, Liu K, Wang Y, Ge X, Ma Y, Qin J, et al. Neurotrophins and neural stem cells in posttraumatic brain injury repair. Animal Model Exp Med. 2024 Feb;7(1):12-23. doi: 10.1002/ame2.12363.
10. Begenisic T, Pavese C, Aiachini B, Nardone A, Rossi D. Dynamics of biomarkers across the stages of traumatic spinal cord injury - implications for neural plasticity and repair. Restor Neurol Neurosci. 2021;39(5):339-366. doi: 10.3233/RNN-211169.
11. Bhardwaj R, Agrawal U, Vashist P, Manna S. Determination of sample size for various study designs in medical research: A practical primer. J Family Med Prim Care. 2024 Jul;13(7):2555-2561. doi: 10.4103/jfmpc.jfmpc_1675_23.
12. Wang L, Tang Z, Li B, Peng Y, Yang X, Xiao Y, et al. Myricetin ameliorates cognitive impairment in 3×Tg Alzheimer's disease mice by regulating oxidative stress and tau hyperphosphorylation. Biomed Pharmacother. 2024 Aug;177:116963. doi: 10.1016/j.biopha.2024.116963.
13. Agraharam G, Girigoswami A, Girigoswami K. Myricetin: a Multifunctional Flavonol in Biomedicine. Curr Pharmacol Rep. 2022;8(1):48-61. doi: 10.1007/s40495-021-00269-2.
14. Sur B, Lee B. Myricetin prevents sleep deprivation-induced cognitive impairment and neuroinflammation in rat brain via regulation of brain-derived neurotropic factor. Korean J Physiol Pharmacol. 2022 Nov 1;26(6):415-425. doi: 10.4196/kjpp.2022.26.6.415.
15. Cebova M, Pechanova O. Protective Effects of Polyphenols against Ischemia/Reperfusion Injury. Molecules. 2020 Jul 30;25(15):3469. doi: 10.3390/molecules25153469.
16. Akram R, Anwar H, Javed MS, Imran A, Rasul A, Malik SA, et al. Natural molecules as promising players against diabetic peripheral neuropathy: an emerging nutraceutical approach. Int J Food Prop. 2023 Sep 22;26(1):894–914. doi: 10.1080/10942912.2023.2189569.
17. Gu SC, Xie ZG, Gu MJ, Wang CD, Xu LM, Gao C, et al. Myricetin mitigates motor disturbance and decreases neuronal ferroptosis in a rat model of Parkinson's disease. Sci Rep. 2024 Jul 2;14(1):15107. doi: 10.1038/s41598-024-62910-6.
18. Li P, Liu J, Wang R, Cao F, Li J, Wang H. Myricetin Mitigated Sevoflurane-induced Cognitive Dysfunction in Aged-mice Through Inhibiting Histone Deacetylase 2/nuclear Factor Erythroid 2-related Factor 2/heme Oxygenase-1 Signalling-mediated Ferroptosis and Mitochondrial Dysfunction. Mol Neurobiol. 2025 Jun;62(6):7776-7791. doi: 10.1007/s12035-025-04703-0.
19. Shen J, Chen S, Li X, Wu L, Mao X, Jiang J, et al. Salidroside Mediated the Nrf2/GPX4 Pathway to Attenuates Ferroptosis in Parkinson's Disease. Neurochem Res. 2024 May;49(5):1291-1305. doi: 10.1007/s11064-024-04116-w.
20. Demyashkin G, Blinova E, Grigoryan M, Parshenkov M, Skovorodko P, Ius V, et al. Neuroprotective Effects of Myricetin on PTZ-Induced Seizures in Mice: Evaluation of Oxidation, Neuroinflammation and Metabolism, and Apoptosis in the Hippocampus. Curr Issues Mol Biol. 2024 Aug 15;46(8):8914-8944. doi: 10.3390/cimb46080527.
21. Calderaro A, Patanè GT, Tellone E, Barreca D, Ficarra S, Misiti F, et al. The Neuroprotective Potentiality of Flavonoids on Alzheimer's Disease. Int J Mol Sci. 2022 Nov 27;23(23):14835. doi: 10.3390/ijms232314835.
22. Jazvinšćak Jembrek M, Oršolić N, Karlović D, Peitl V. Flavonols in Action: Targeting Oxidative Stress and Neuroinflammation in Major Depressive Disorder. Int J Mol Sci. 2023 Apr 7;24(8):6888. doi: 10.3390/ijms24086888.
23. Szulc A, Wiśniewska K, Żabińska M, Gaffke L, Szota M, Olendzka Z, et al. Effectiveness of Flavonoid-Rich Diet in Alleviating Symptoms of Neurodegenerative Diseases. Foods. 2024 Jun 19;13(12):1931. doi: 10.3390/foods13121931.
24. Sethiya NK, Ghiloria N, Srivastav A, Bisht D, Chaudhary SK, Walia V, et al. Therapeutic Potential of Myricetin in the Treatment of Neurological, Neuropsychiatric, and Neurodegenerative Disorders. CNS Neurol Disord Drug Targets. 2024;23(7):865-882. doi: 10.2174/1871527322666230718105358.
25. Wang S, Zhang J. Oxidative Damage and Anti-Inflammatory Activity of Myricetin from Berries in Streptozotocin-Induced Diabetic Rats. Int. J. Pharmacol. 2022 May 1;18(4):753–64. doi: 10.3923/ijp.2022.753.764
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Pakistan Journal of Medicine and Dentistry
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open-access article distributed under the terms of the CreativeCommons Attribution License (CC BY) 4.0 https://creativecommons.org/licenses/by/4.0/
Metrics
How to Cite
